Picolinic acid: a mobile phase additive for improved chromatography of metal-chelating heterocyclic acids and β-diketones

✍ Scribed by D.W. Roberts; R.J. Ruane; I.D. Wilson

• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 332 KB
• Volume: 471
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94191-8

✦ Synopsis

During the course of the development of high-performance liquid chromatographic (HPLC) analytical methods for some metal-chelating compounds from two distinct chemical series, one series containing a heterocyclic acid, the other a b-diketone (see Fig. ! for general structures), severe problems were encountered due to poor chromatographic peak shapes. Thus, using reversed-phase HPLC, peaks from these compounds were characterised by excessive tailing, peak asymmetry and, in the worst cases, "chair''-shaped peaks. These problems were clearly a property of the compounds under investigation and did not reflect deficiencies in the instrumentation.