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Pichiasurface display: display of proteins on the surface of glycoengineeredPichia pastorisstrains

โœ Scribed by Pieter P. Jacobs; Stefan Ryckaert; Steven Geysens; Kristof De Vusser; Nico Callewaert; Roland Contreras

Publisher
Springer Netherlands
Year
2008
Tongue
English
Weight
351 KB
Volume
30
Category
Article
ISSN
0141-5492

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A novel system (DBDX) was developed which allows the external surface display on filamentous bacteriophage of proteins fused to either the N- or the C-terminus of a DNA-binding protein. In conjunction with helper phage infection, expression of proteins fused to the estrogen receptor DNA-binding doma