Pichia Protocols Volume 103 || Transformation

✍ Scribed by Higgins, David R.; Cregg, James

• Book ID: 120325689
• Publisher: Humana Press
• Year: 1998
• Tongue: English
• Weight: 117 KB
• Edition: 1
• Category: Article
• ISBN-13: 9780896034211
• DOI: 10.1385/0-89603-421-6:27

✦ Synopsis

Introduction To Pichia Pastoris / David R. Higgins, James M. Cregg -- Classical Genetic Manipulation / James M. Cregg, Shigang Shen, Monique Johnson, Hans R. Waterham -- Transformation / James M. Cregg, Kimberly A. Russell -- Small Vectors For Expression Based On Dominant Drug Resistance With Direct Multicopy Selection / David R. Higgins, Katie Busser, John Comiskey, Peter S. Whittier, Thomas J. Purcell, James P. Hoeffler -- The Generation Of Multicopy Recombinant Strains / Mike Romanos, Carol Scorer, Koti Sreekrishna, Jeff Clare -- Isolation Of Nucleic Acids / David J. Miles, Katie Busser, Christine Stalder, David R. Higgins -- Generation Of Protease-deficient Strains And Their Use In Heterologous Protein Expression / Martin A. Gleeson, Christopher E. White, David P. Meininger, Elizabeth A. Komives -- Glycosylation Profiling Of Heterologous Proteins / José A. Cremata, Raquel Montesino, Omar Quintero, Rossana García -- High Cell-density Fermentation / Jayne Stratton, Vijay Chiruvolu, Michael Meagher -- Use Of Pichia Pastoris As A Model Eukaryotic System : Peroxisome Biogenesis / Klaas Nico Faber, Ype Elgersma, John A. Heyman, Antonius Koller, Georg H. Lüers, William M. Nuttley, Stanley R. Terlecky, Thibaut J. Wenzel, Suresh Subramani -- Secretion Of Recombinant Human Insulin-like Growth Factor I (igf-i) / Russell A. Brierley -- Secretion Of Scfv Antibody Fragments / Hermann Gram, Rita Schmitz, Rüdiger Ridder -- Expression Of Tetanus Toxin Fragment C / Jeff Clare, Koti Sreekrishna, Mike Romanos -- Expression Of Egf And Hiv Envelope Glycoprotein / Jeff Clare, Carol Scorer, Rich Buckholz, Mike Romanos -- Expression Of An Integral Membrane Protein, The 5ht5a Receptor / H. Markus Weiss, Winfried Haase, Helmut Reiländer -- Localization Of The Myc-tagged 5ht5a Receptor By Immunogold Staining Of Ultrathin Sections / Winfried Haase, H. Markus Weiss, Helmut Reiländer -- Foreign Proteins Expressed In P. Pastoris. Edited By David R. Higgins And James M. Cregg. Includes Bibliographical References And Index.