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Pichia pastorisfermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement

โœ Scribed by Wenhui Zhang; Karen J. Hywood Potter; Bradley A. Plantz; Vicki L. Schlegel; Leonard A. Smith; Michael M. Meagher

Book ID
106308910
Publisher
Springer-Verlag
Year
2003
Tongue
English
Weight
275 KB
Volume
30
Category
Article
ISSN
1476-5535

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Continuous fermentation was applied to the production of recombinant human chymotrypsinogen B (hCTRB) by the methylotrophic yeast Pichia pastoris as a tool for the kinetic analysis of growth and product formation. Using methanol as the sole source of carbon, energy, and induction, cell growth could