Phytoglycoprotein (38 kDa) induces cell cycle (G0/G1) arrest and apoptosis in HepG2 cells

✍ Scribed by Jin Lee; Kye-Taek Lim

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 487 KB
• Volume: 112
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.23239

✦ Synopsis

Abstract

Styrax japonica Siebold et al Zuccarini (SJSZ) has been used to heal inflammation and bronchitis as folk medicine in Korea. Firstly, glycoprotein isolated from SJSZ (SJSZ glycoprotein) has a molecular weight with 38 kDa and consists of carbohydrate (57.64%) and protein (42.35%). In the composition of SJSZ glycoprotein, carbohydrate mostly consists of glucose (28.17%), galactose (21.85%), and mannose (2.62%) out of 52.64%, respectively. The protein consists of Trp (W, 7.01%), Pro (P, 6.72%), and Ile (I, 5.42%) out of 42.35% as three major amino acids, while total amount of other amino acids is 23.20%. The purpose of this study is to know whether the SJSZ glycoprotein (38 kDa) induces the cell cycle arrest and apoptosis in HepG2 cells. Cytotoxicity was evaluated using MTT and lactate dehydrogenase assay and amount of intracellular reactive oxygen species (iROS) and nitric oxide (NO) was measured using fluorescence microplate reader. Activities of cell cycle‐related proteins [p53, p21, p27, Cyclin D1, and cyclin‐dependent kinase (CDK)4] and apoptosis‐related factors [iNOS, Bid, Bcl‐2/bax, cytochrome c, caspase‐9, caspase‐3, and poly‐(ADP‐ribose) polymerase (PARP)] were assessed by Western blot and fluorescence‐activated cell sorter (FACS) analysis. In the cell cycle‐related proteins, SJSZ glycoprotein (50 µg/ml) significantly enhances the expression of p53, p21, and p27, whereas it suppressed the activity of cyclin D1/CDK4. In the apoptosis‐related factors, SJSZ glycoprotein (50 µg/ml) stimulates to increase iROS, and NO, to activate iNOS, Bid, Bcl‐2/bax, cytochrome c, caspase‐9, caspase‐3, and PARP. SJSZ glycoprotein (50 µg/ml) has potent effect to arrest cell cycle from G~0~/G~1~ to S and to induce apoptosis in HepG2 cells. J. Cell. Biochem. 112: 3129–3139, 2011. © 2011 Wiley Periodicals, Inc.