Three GTPβbinding proteins with apparent molecular masses of 27, 28 and 30 kDa have been detected in isolated nuclei of etiolated pea plumules. After LDSβPAGE and transfer to nitrocellulose these proteins bind [^32^p]GTP in the presence of excess ATP, suggesting that they are monomeric G proteins. W
Phytochrome and calcium stimulation of protein phosphorylation in isolated pea nuclei
β Scribed by Neeraj Datta; Yuh-Ru Chen; Stanley J. Roux
- Book ID
- 115758734
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 477 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0006-291X
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Calcium-dependent protein phosphorylation may be a critical step in the stimulated secretion of anterior pituitary hormones. We have noted the existence of a number of calcium-calciudcalmodulin-, and calciudphospholipid-dependent phosphoproteins in the normal rat anterior pituitary. Cell extracts we