Physiology of polyol formation by free and immobilized cells of the osmotolerant yeastPichia farinosa

✍ Scribed by Ute Höötmann; Bernward Bisping; Hans-Jürgen Rehm

• Book ID: 104654895
• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 531 KB
• Volume: 35
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00184698

✦ Synopsis

Some physiological data o f cells o f Pichia farinosa immobilized on sintered glass Raschig rings were c o m p a r e d with data from free cells. Glucose consumption and productivity of total polyols (arabitol, glycerol and erythritol) showed a simultaneous "interlag" phase. Enzymes that catalyse steps o f the pentosephosphate pathway (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, transaldolase and polyol dehydrogenase) showed a distinct increase after transfer o f the cells into production medium. The activity o f glycerol-3-phosphate dehydrogenase was generally low. Only alcohol dehydrogenase presented the inter-lag phase mentioned above.