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Physiological aspects of conjugation in fission yeast

✍ Scribed by R. Egel

Publisher
Springer-Verlag
Year
1971
Tongue
English
Weight
454 KB
Volume
98
Category
Article
ISSN
0032-0935

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✦ Synopsis

Conjugation was studied in Schizosaccharomyces pombe using liquid media. Nitrogen, which was growth-limiting in a synthetic medium, had to be consumed completely before conjugation could start. Conjugation was preceded by sexual agglutination. Agglutinability was not constitutive in heterothallie strains. It only developed when cells of h + and h-mating type were grown in mixed culture for at least 2.5 hr before the start of conjugation.

πŸ“œ SIMILAR VOLUMES

Temperature sensitivity of flocculation
Temperature sensitivity of flocculation induction, conjugation and sporulation in fission yeast
✍ G. B. Calleja; B. F. Johnson πŸ“‚ Article πŸ“… 1979 πŸ› Springer Netherlands 🌐 English βš– 477 KB

Homothallic cultures of Schizosaccharomyces pombe, anaerobically grown to stationary phase in broth at 32 degrees C, were induced by aeration to flocculate. Flocculation was followed by copulation, conjugation, zygote formation, meiosis and sporulation. Cultures grown to stationary phase at 32 degre