Physicochemical Study of Several Peptide Constructs Based on the Sequence (96–107) of VP2-HAV Protein
✍ Scribed by F.M. Mota; M.A. Busquets; F. Reig; M.A. Alsina; I. Haro
- Book ID
- 102579308
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 368 KB
- Volume
- 188
- Category
- Article
- ISSN
- 0021-9797
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✦ Synopsis
A peptide with the amino acid sequence of 96-107 of Hepatitis vated HAV vaccine is available (2). Although it is highly A virus capsid protein VP2 was synthesized as such (FR2), as immunogenic, the cost involved in its production does not stearoyl derivative (stearoyl-FR2), and as a multiple antigen demake it viable for vaccination campaigns in developing rivative (MAP-FR2) by standard solid phase procedures. The countries. With the aim to reduce the production expenses, products were characterized by HPLC, mass spectrometry, and a new generation of peptidic vaccines is being developed. amino acid analysis. Their surface properties were studied using In this sense, peptide fragments, enclosing epitopes, have Langmuir films. Free peptide as well as its derivatives showed a shown their ability to generate antibodies capable of recoghigh surface activity giving surface pressure increases in a concennizing and neutralizing viral particles (3, 4). tration dependent way. FR2 molecules reached the air/water interface within few minutes. In contrast, stearoyl-FR2 and MAP 4 -FR2
Despite the effectiveness of these peptides to induce imshowed an induction time concentration dependence, suggesting munological response, their effect is in general lower than the presence of aggregates. These products were also able to insert that reached with proteins and intact virus. In order to pointo dipalmitoyl phosphatidyl choline (DPPC), dipalmitoyl phostentiate antibody production by peptides, several attempts phatidyl ethanolamine (DPPE) and dipalmitoyl phosphatidyl have been done. One of them consists on the peptide conjuglycerol (DPPG) monolayers spread at 5, 10, and 20 mN m 01 . gation by means of a condensation agent, with a known Miscibility studies of the peptides with phospholipids showed no protein (BSA, KLH) or with a synthetic polymeric carrier deviation with respect to ideality, which suggests the presence of (5) that behave as adjuvants. Another possibility, is the comweak molecular interactions. Finally, FR2 was incorporated into bination of the peptide with an adjuvant (Freund's adjuvant) liposomes in order to study the immunogeneicity and surface activwithout the conjugation step mentioned above. In both cases, ity of these vesicles. ᭧ 1997 Academic Press an antigenic macromolecular structure is obtained. Although