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Physicochemical studies on xylinan (acetan). III. Hydrodynamic characterization by analytical ultracentrifugation and dynamic light scattering

✍ Scribed by Stephen E. Harding; Gisela Berth; Jürgen Hartmann; Kornelia Jumel; Helmut Cölfen; Bjørn E. Christensen

Publisher
Wiley (John Wiley & Sons)
Year
1998
Tongue
English
Weight
666 KB
Volume
39
Category
Article
ISSN
0006-3525

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✦ Synopsis

A laboratory-made sample of the polysaccharide xylinan (acetan) has been further characterized with respect to (i) purity, (ii) molar mass and polydispersity, and (iii) gross conformation by a combination of hydrodynamic measurements (sedimentation velocity and equilibrium analytical ultracentrifugation, viscometry, and dynamic light scattering) in aqueous NaCl (I = 0.10 mol. L-'). Sedimentation velocity diagrams recorded using Schlieren optics revealed highlypure material sedimenting as a single boundary [ s ; ~, ~ = 9.5 +-0.7) S; k, = (273 k 112) rnL/g]. The hypersharp nature of these boundaries is symptomatic ofa polydisperse and highly nonideal (in the thermodynamic sense) system. Low speed sedimentation equilibrium in the analytical ultracentrlfuge using Rayleigh interference optics and two diflerent types of extrapo-

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Physicochemical studies on xylinan (acet
Physicochemical studies on xylinan (acetan). II. Characterization by static light scattering
✍ Gisela Berth; Herbert Dautzenberg; Bjørn E. Christensen; Olav Smidsrød 📂 Article 📅 1998 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 577 KB

Laboratory-made samples of the polysaccharide xylinan, also called acetan, were studied in aqueous solution at various ionic strengths I (0.01 mol/L I I 5 0.30 mol/L). The conditions for clarification (ultracentrijugation/membranejiltration) were studied. The Zimm procedure was used to obtain the av

Physicochemical studies on xylinan (acet
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Laboratory-made samples of the polysaccharide xylinan (acetan) were fiactionated on Sepharose Cl-2B using O.IM NaCl as eluant. The weight average molar masses and intrinsic viscosities were estimaled in the fractions by multiangle laser light scattering (oflline) and capillary viscometPy, respective