Effect of an E461G Mutation of β-Galacto
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John P. Richard; Reuben E. Huber; Deborah A. McCall
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Article
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2001
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Elsevier Science
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English
⚖ 116 KB
An E461G mutation of -galactosidase results in the disappearance of the high pL (L ϭ H, D) downward break in the rate profiles for k cat /K m for wild-type enzyme-catalyzed hydrolysis of 4-nitrophenyl -D-galactopyranoside (Gal-OPNP) and a decrease from (k cat ) HOH /(k cat ) DOD ϭ 1.7 to (k cat )