Physicochemical and biological studies of corona-treated artificial membranes used for pancreatic islets encapsulation: Mechanism of diffusion and interface modification

✍ Scribed by Kessler, L. ;Legeay, G. ;West, R. ;Belcourt, A. ;Pinget, M.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 534 KB
• Volume: 34
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(199702)34:2<235::aid-jbm13>3.0.co;2-h

✦ Synopsis

The artificial AN69 membrane (Hospal), a synthetic copoly-of glycerol molecules at the membrane surface and from a mer composed of acrylonitrile and sodium methallyl sulpho-biological point of view, produced a threefold increase in nate suitable for pancreatic islet encapsulation, was submit-insulin diffusion. Furthermore, the biocompatibility of the ted to physicochemical treatment (Corona discharge) to treated membrane was preserved after 1 year of intraperitoimprove its insulin permeability. X-ray photoelectron spec-neal implantation. The increase in insulin permeability troscopy (XPS) analysis of the AN69 membrane indicated should result from a decrease of the membrane polarity and the presence of up to two molecular layers of glycerol at its of a steric hindrance in pores. Thus, Corona discharge treatsurface while the surface energies revealed the presence of ment may serve to optimize the properties of artificial memhydrophilic sites (ϪSO 3 Na/glycerol) located at the mem-branes used for pancreatic islets encapsulation. ᭧ 1997 John brane surface and acrylonitrile hydrophobic groups inside