Physicochemical and Biological Characterization of Polyethylenimine-graft-Poly(ethylene glycol) Block Copolymers as a Delivery System for Oligonucleotides and Ribozymes
β Scribed by Brus, Carola; Petersen, Holger; Aigner, Achim; Czubayko, Frank; Kissel, Thomas
- Book ID
- 126906727
- Publisher
- American Chemical Society
- Year
- 2004
- Tongue
- English
- Weight
- 193 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1043-1802
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## Abstract An ideal gene carrier is required both in safety and efficiency for transfection. Polyethylenimine (PEI), a wellβstudied cationic polymer, has been proved with high transfection efficiency, but is reported as toxicity in many cell lines. In this study, PEI was coupled with polyethylene
Hyper-branched polyethylenimine grafted polycaprolactone block mono-methoxyl poly (ethylene glycol) copolymer (hy-PEI-g-PCL-b-mPEG) was obtained through the conjugation of mPEG-PCL with hyperbranched PEI (hy-PEI) based on the Michael addition. mPEG-PCL was synthesized by ring-opening polymerization