✦ LIBER ✦

Physical studies of the interaction between detergent-solubilized cholesterol and PC vesicles

✍ Scribed by C.F. Schmidt; J.K. Chun; A.V. Broccoli; R.P. Taylor

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 386 KB
Volume: 22
Category: Article
ISSN: 0009-3084
DOI: 10.1016/0009-3084(78)90038-5

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✦ Synopsis

The R3C NM R spectrum of [4-13C] cholesterol solubilized in aqueous solutions of sodium dodecyl sulfate indicates that the molecule has considerably more rotational freedom than when it is intercalated into phosphatidylcholine (PC) vesicles. The interaction between detergent-solubilized cholesterol and PC vesicles has been studied by both NMR and gel permeation chromatography. Our results indicate that greater that 90% of the cholesterol is slowly transferred into the PC bilayer when the detergent-solubilized cholesterol is mixed with PC vesicles.

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