Physical Responses of Yeast Cells to Osmotic Shock
β Scribed by ROSE, D.
- Book ID
- 114734157
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 1975
- Tongue
- English
- Weight
- 374 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0021-8847
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Shock exposure of osmotolerant yeasts Debaryomyces hansenii 3339, Zygosaccharomyces mellis 3296 and Kloeckera anfilarrum 3046 to solutions of sucrose at water activity (Aw) values between 0.99 and 0.88 was found to cause a great decrease in cell volume but an increase in heat resistance. For Debaryo
Yeast cells were permeabilized by incubation in 0.8 M sorbitol followed by suspension in dilute buffer. A preincubation with 2-mercaptoethanol was also included for optimal permeabilization. More than 90% of the treated cells were stainable with methylene blue. Determinations of cell wall-synthesizi