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Physical mapping of the brain and ovarian aromatase genes in the Nile Tilapia, Oreochromis niloticus, by fluorescence in situ hybridization

✍ Scribed by S. C. Harvey; J. Y. Kwon; D. J. Penman

Publisher: John Wiley and Sons
Year: 2003
Tongue: English
Weight: 71 KB
Volume: 34
Category: Article
ISSN: 0268-9146
DOI: 10.1046/j.1365-2052.2003.00941.x

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✦ Synopsis

Summary

Cytochrome P450‐aromatase enzyme (CYP19), which catalyses the conversion of androgens to oestrogens, is critical in ovarian differentiation and hence in the sex differentiation pathways of non‐mammalian vertebrates. As in other fish species, distinct ovarian and brain aromatase genes have been identified in the Nile Tilapia, Oreochromis niloticus. Here we demonstrate by in situ hybridization that the two aromatase genes of this species are present on different chromosomes and that neither are located on the sex chromosomes. Hence, the aromatase genes are not the primary sex determination genes in O. niloticus.

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