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Physical map of chloroplast DNA of aerial yam,Dioscorea bulbiferaL.

โœ Scribed by R. Terauchi; T. Terachi; K. Tsunewaki

Publisher
Springer
Year
1989
Tongue
English
Weight
885 KB
Volume
78
Category
Article
ISSN
0040-5752

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โœฆ Synopsis

A physical map of chloroplast DNA (ctDNA) of aerial yam, Dioscorea bulbifera L. was constructed using three restriction endonucleases, PstI, SalI, and SmaI. In addition, a clone bank of the BamHI-digested fragments were generated, and the locations of most BamHI fragments on the map were also determined. The ctDNA of D. bulbifera was found to be a circular molecule with a total size of ca. 152 kb involving two inverted repeats of ca. 25.5 kb, and small and large single copy regions of ca. 18.5 and 83.4 kb, respectively. The genes for the large subunit of the ribulose 1,5-bisphosphate carboxylase (rbcL) and the ATP-synthase subunits ฮฒ and ษ› (atpB/atpE) were mapped.

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