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Physical linkage of the SLG and SRK genes at the self-incompatibility locus of Brassica oleracea

✍ Scribed by Boyes, Douglas C. ;Nasrallah, June B.

Book ID: 104699619
Publisher: Springer
Year: 1993
Tongue: English
Weight: 873 KB
Volume: 236-236
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00277135

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✦ Synopsis

In Brassica oleracea, the pollen-stigma interaction of self-incompatibility is controlled by a single genetically defined locus designated S. Molecular studies have identified two genes that are tightly linked to the classically defined S locus: The S-Locus Glycoprotein (SLG) gene and the S-Receptor Kinase (SRK) gene. In previous RFLP linkage analyses with probes specific for SLG and SRK, we were unable to identify any recombination events between SLG, SRK, and self-incompatibility phenotype. In this paper, we use pulsed-field gel electrophoresis (PFGE) in conjunction with DNA blot analysis to characterize the S-locus region from two highly divergent self-incompatibility genotypes, S2 and S6. We establish the physical linkage of SLG and SRK in each genotype, and demonstrate that the two genes are separated by a maximum distance of 220 kb in the S6 genotype and 350 kb in the S2 genotype. Furthermore, a comparison of the data from the two genotypes reveals that a high level of polymorphism exists across the entire S-locus region.

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