Physical immobilization characteristics of a hybridoma in a glass bead packed-bed reactor

✍ Scribed by O. T. Ramírez; R. Mutharasan

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 371 KB
• Volume: 33
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260330816

✦ Synopsis

One of the important considerations in design of large scale cell culture systems is the fragility or shear-sensitivity of animal cells. The engineering approach to overcome this problem has been to entrap the cells in various physical structures, such as hollow fibers, microencapsulation, fluidized sponge beads, and others. Packed-bed configurations not only can retain cells in the inter-particle voids, but could potentially provide the needed shear protection to animal cells. Even though packed-bed systems have been successfully used for bacterial and yeast strains, only a few reports exist on its use for cultivating surface independent animal These rely on ceramic packings that can strongly adsorb the animal cells onto their surface. Other packing materials such as glass beads have been used extensively but its application has been limited to only anchorage dependent cells .6-9

Recent laboratory work showed that a glass bead packedbed reactor can be used for the cultivation of a surface independent animal cell. ' ' The initial work with hybridoma * To whom all correspondence should be addressed.