โ Scribed by Schachman, H. K.
No coin nor oath required. For personal study only.
It is rather difficult for me to have anticipated everything that Doctor Doty was going to talk about; and, not having had tlic pleasure of attending any of the Oak Ridge Syniposia, I have no precedence on whicli to base my activities iii the role of a discussant. I will t r y to arrange rriy remarks in an o~-der corresponding to Doty ' s talk.
TVhrn tJ. D. Watson and F. H. C. Crick first proposed their exciting structural model for deoxyribonucleic acid (DNA) , we thought it should be possible to obtain information to test this model from a study of the l<inetics of tlic enzyrriic degradation of DNA. Since the enzymologists havc not yet fourid much specificity for deoxyribonuclease (DNAse) , we hope that the enzyme is behaving the way we want it to hehavenamely, that it is breaking only phosphodiester bonds, one at a time and also indiscriminately. There is cause for concern here because only about one in every five bonds is broken after complete digestion by the enzyme, and yet we are assuming that the enzyme breaks bonds at random. W e would like to examine the decrease in molecular weight as a function of time of enzyme treatment and to determine when and how the molecule begins to fall apart. Our data 'The work reported here has been supported by a grant from the National Rcienee Foundation and has been performed largely with V. N. Schumaker and E. Glen Riehards.
๐ SIMILAR VOLUMES
## Abstract Measurements have been made of the intensity of light scattered from aqueous solutions of calf thymus DNA with and without the application of electric fields. For fields approaching 150 V/cm and frequencies below 2.5 KHz, changes (ฮ__I__) of up to 10% in the residual scattered intensity
## Synopsis $elutions of calf thymus NaRNA in 0.2M NaCl and CsDNA in 0.2M CsCl (fragmented by sonic irradiation to a molecular weighb of about 5 X 106) were examined by electron microscopy, light scattering, and viscosity measurements. Electron microscopy showed that the molecular fragments are ro