Physical and biological stability of dehydro-thermally crosslinked α-amylase-poly(vinyl alcohol) blends

✍ Scribed by Cristallini, Caterina; Barbani, Niccoletta

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 385 KB
• Volume: 47
• Category: Article
• ISSN: 0959-8103
• DOI: 10.1002/(sici)1097-0126(199812)47:4<491::aid-pi93>3.0.co;2-e

✦ Synopsis

A dehydro-thermal treatment has been used to crosslink a-amylase (EC 3.2.1.1)Èpoly(vinyl alcohol) blends in order to reduce their water solubility and to favour their potential use as bio-artiÐcial polymeric materials based on enzymes. The inÑuence of a thermal treatment on the thermal and biological stability of the enzyme blended with a synthetic polymer was carefully studied by means of calorimetry and biological assays. The values of the kinetic parameters obtained from the biological assays indicate that the enzyme speciÐcity for the substrate in all the blends examined is not a †ected by the thermal treatment. In addition, the results of the releasing tests showed that the thermally crosslinked a-amylaseÈpoly(vinyl alcohol) blends can be considered suitable for manifacturing delivery systems able to release enzymes.