Although the genetic code is defined by a linear array of nucleotides, it is the three-dimensional structure of the double helix that regulates most of its cellular functions. Over the past two decades, it has hecome increasingly clear that aspects of this three-dimensionality which reflect topologi
Phylogenomics of type II DNA topoisomerases
✍ Scribed by Danièle Gadelle; Jonathan Filée; Cyril Buhler; Patrick Forterre
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 424 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0265-9247
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Type II DNA topoisomerase activity is required to change DNA topology. It is important in the relaxation of DNA supercoils generated by cellular processes, such as transcription and replication, and it is essential for the condensation of chromosomes and their segregation during mitosis. In mammals
## Abstract The sensitivity of HeLa DNA topoisomerase II to 45°C heat shock was measured both in the intact cell and in vitro. In the intact cell, DNA topoisomerase II activity was estimated by measuring the formation and reversal of enzyme‐DNA cleavable complexes by alkaline filter elution of cell