Photothermolysis of blood vessels using indocyanine green and pulsed diode laser irradiation in the dorsal skinfold chamber model
✍ Scribed by Philipp Babilas; Gal Shafirstein; Jürgen Baier; Vivien Schacht; Rolf-Markus Szeimies; Michael Landthaler; Wolfgang Bäumler; Christoph Abels
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 255 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0196-8092
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✦ Synopsis
Abstract
Background and Objective
For the treatment of vascular lesions, the use of laser light absorbed by the endogenous chromophore hemoglobin may still be improved.
Materials and Methods
Laser treatment (λ~em~ = 805 nm; fluence rate: 106 kW/cm^2^; fluence: 3.2 J/cm^2^ (3 milliseconds)), of blood vessels directly after i.v. application of indocyanine green (ICG) (ICG‐concentration: 0, 2, or 4 mg/kg body weight (b.w.)) (n = 14,117) was investigated in the skinfold chamber model. Vessel diameters (1–351 µm) were measured using intravital fluorescence microscopy up to 24 hours following irradiation. Histology was taken 1 or 24 hours after irradiation. Results were compared to a mathematical model based on the finite element method.
Results
The reduction of blood vessel perfusion was proportional to ICG‐concentration and pulse duration; only a 30 milliseconds pulse duration (2 or 4 mg/kg b.w. ICG‐concentration) induced a loss of perfusion even of blood vessels with a diameter <30 µm. Histology revealed photocoagulation of blood vessels up to 24 hours. Results were in agreement with mathematical calculations.
Conclusion
ICG‐mediated laser irradiation induces irreversible photocoagulation of blood vessels of all diameters in this model. Lasers Surg. Med. 39: 341–352, 2007. © 2007 Wiley‐Liss, Inc.