Photothermal image cytometry of human neutrophils

✍ Scribed by Dmitry Lapotko; Georgy Kuchinsky; Michael Potapnev; Dmitry Pechkovsky

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 580 KB
• Volume: 24
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/(sici)1097-0320(19960701)24:3<198::aid-cyto2>3.0.co;2-e

✦ Synopsis

Photothermal imaging, when applied to the study of living cells, provides morphofunctional information about cell populations. In technical terms, the method is complementary to optical microscopy.

The photothermal method was used for cell imaging and quantitative studies. We have developed a dual-laser technique and have constructed an automated image cytometer based on an optical microscope. The theory of the method, the technical equipment, and the computer software are de-Laser-tissue interaction is used in various cytometric applications such as flow cytometry, laser microscopy, etc. When laser radiation is applied to samples such as single cells, the absorption of light is considered to be the most common process. It may lead to heating of the sample due to nonradiational relaxation, fluorescence and phosphorescence, and photophysical and photochemical phenomena. The thermal effects are recognized as the most common in the case of laser action on viable (nonstained) tissue or cells (2,10,12,15). However, studies of the thermal effects of laser irradiation at the level of a single cell are rather limited (2,9) compared with the studies of thermal phenomena in larger samples (6-8) or