Photothermal detection of laser-induced damage in single intact cells

✍ Scribed by Dmitri O. Lapotko; Ekaterina Y. Lukianova; Alexander I. Shnip

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 217 KB
• Volume: 33
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/lsm.10285

✦ Synopsis

Abstract

Background and Objective

Most of the studies of laser‐induced damage do not analyze individual cells. Objective of this work was to evaluate local photo‐induced thermal phenomena in single cells at theoretical and experimental levels for developing the method for real‐time detection of laser damage in intact cells.

Study Design/Materials and Methods

Theoretical model of cell–laser interaction assumes local nature of photo‐induced thermal effects and describes photodamage through bubble formation. Photothermal (PT) method was suggested for damage detection. Laser‐induced damage was verified for individual cells with two techniques through detection of Trypan blue penetration into damaged cell.

Results

Specific PT responses from blast‐transformed lymphocytes were identified independently as result of bubble formation and cell damage. Probability of cell damage was obtained for cells as function of laser pulse energy.

Conclusions

The Laser load test (LLT) was suggested for real‐time detection of damage, damage threshold measurement, and investigation of intact single cells. Lasers Surg. Med. 33:320–329, 2003. © 2003 Wiley‐Liss, Inc.