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Photosystem II characteristics of a constructedSynechocystis6803 mutant lacking synthesis of the D1 polypeptide

✍ Scribed by Fredrik Nilsson; Bertil Andersson; Christer Jansson

Book ID: 104615325
Publisher: Springer
Year: 1990
Tongue: English
Weight: 271 KB
Volume: 14
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00019402

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✦ Synopsis

Photosystem II (PSII) composition was studied in a mutant of the cyanobacterium Synechosystis 6803 in which synthesis of the reaction center polypeptide D 1 has been inactivated. The mutant thylakoids had lost also the other reaction center polypeptide D2 and the chlorophyll a-binding protein CP47. Cytochrome b559 and the chlorophyll a-binding protein CP43 accumulated to almost wild-type amounts in mutant thylakoids. Also the 33 kDa polypeptide involved in water oxidation was present and membrane-bound in mutant thylakoids. The intrinsic 22 kDa polypeptide, so far known only from plants, was detected both in wild-type and mutant thylakoids.

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