Photosynthetic and dark carbon metabolism in unicellular blue-green algae

✍ Scribed by Pelroy, R. A. ;Bassham, J. A.

• Publisher: Springer-Verlag
• Year: 1972
• Weight: 739 KB
• Volume: 86
• Category: Article
• ISSN: 0003-9276
• DOI: 10.1007/bf00412397

✦ Synopsis

1. The kinetics of ~tCO 2 incorporation into cellular intermediates was used to determine the primary pathway of carbon fixation by four genetically diverse unicellular blue-green algae. In each case label was first detected in 3-phosphoglycerate and then in compounds of the reductive pentose cycle.

2. A light to dark transition evoked the same response in all four strains: Immediate cessation of biosynthesis, rapid increase in the concentration of 6-phosphoglueonate and changes in the concentrations of sugar mono-and diphosphates. On the other hand, after the first few seconds of dark incubation little or comparatively slow change was noted in the concentrations of 3-phosphoglycerate, phosphoenolpyruvate, citrate, aspartate, and glutamate.

3. For one strain (Aphanocapsa 6308) an experiment using both a2p and 14C0~