Photosynthetic ability in dark-grownReboulia hemisphaericaandBarbula unguiculatacells in suspension culture

✍ Scribed by Susumu Takio; Chikako Akita; Victoria Wambui Ngumi; Shinji Takami

• Book ID: 104694585
• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 523 KB
• Volume: 8
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00270056

✦ Synopsis

Suspension cultured cells of the liverwort, Reboulia hemisphaerica and of the moss, Barbula unguiculata were independently subcultured in the medium containing 2% glucose in the dark or in the light for more than one year, and the photosynthetic activities of the final cultures were determined. Throughout the culture period light-grown cells of both species contained high amount of chlorophyll (4 to 34 μg mg(-1) dry weight) and showed a high photosynthetic activity (10 to 84 μmol O2 mg(-1) chlorophyll h(-1)). Dark-grown cells of R. hemisphaerica showed the same level of chlorophyll content and photosynthetic O2 evolving activity as light-grown cells. Although chlorophyll content in dark-grown B. unguiculata cells was ten-fold lower than that in light-grown cells, the photosynthetic activity of these dark-grown cells was higher than that of light-grown cells based on chlorophyll content.