Photostimulation of two types of Ca2+ waves in rat pheochromocytoma PC12 cells by ultrashort pulsed near-infrared laser irradiation
✍ Scribed by N. I. Smith; S. Iwanaga; T. Beppu; K. Fujita; O. Nakamura; S. Kawata
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 283 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1612-2011
No coin nor oath required. For personal study only.
✦ Synopsis
We show that focused laser irradiation can generate
Ca^2+^ waves in differentiated PC12 cells, using highly
penetrating and locally acting near-infrared femtosecond pulsed
laser light. Similar to photolytic uncaging experiments,
intracellular Ca^2+^ concentrations could be elevated to
produce a whole cell Ca^2+^ rise, but without the requirement
for caged Ca^2+^. Irradiation of subcellular volumes in the
soma or in PC12 neurites generated a relatively slow calcium rise
(speed ∼20 μm/s). Less frequently, a fast calcium rise
(speed >1200 μm/s) was observed, consistent with laser-induced
membrane depolarization. The probability of Ca^2+^ generation
increased with laser power and was found to be the same in
excitable (PC12) and non-excitable (HeLa) cells.