Photoreversal of ultraviolet injury to frog and crab nerves

✍ Scribed by Pierce, Stirling ;Giese, Arthur C.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1957
• Tongue: English
• Weight: 661 KB
• Volume: 49
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030490212

✦ Synopsis

FIVE FIGURES

Ultraviolet (UV) injury as manifested in suppression of cell division, o r induction of mutations, is considered to be largely a nuclear phenomenon presumably resulting from suppression of desosyribonucleic acid (DNA) synthesis wliich is limited to the nucleus. Photoreactivation appears to be tlie reversal of the suppression of DNA synthesis (Kelner, '53). Conversely, the effect of UV on ciliary movement which is a strictly cytoplasmic phenomenon appears to be very little if at all subject to photoreversal (Brandt and Giese, '56). Consequently it might be predicted that injurious effects of UV upon the nerve fiber lacking a nucleus should show no photoreversal. The experiments reported below indicate that UV injury to nerves is, in fact, photoreversed. Supported in part by Contract AP(l1-1234) with the Atomic Energy Commission; in part, during the terminal stage, by Public Health Contract C-l799(C2). We are indebted to Dr. F. Crescitelli of the University of California a t LOS Angeles f o r suggesting methods on stripping nerves, to Dr. T. Bullock of the same university for suggrstions on the use of a perfusion chamber i n which tlie nerve was irradiated and for initially suggesting this line of experimentation, to Dr. C. L. Prosser of the University of Illinois f o r suggestions of ionic tests, and to Dr. L. R. Blinks for making available facilities for this work a t the Hopkills Marine Station during the summer of 1931. Preliminary reports on this work have appeared in abstracts (