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Photophysics and Dihedral Freedom of the Chromophore in Yellow, Blue, and Green Fluorescent Protein

✍ Scribed by Megley, Colleen M.; Dickson, Luisa A.; Maddalo, Scott L.; Chandler, Gabriel J.; Zimmer, Marc


Book ID
125996178
Publisher
American Chemical Society
Year
2009
Tongue
English
Weight
737 KB
Volume
113
Category
Article
ISSN
0022-3654

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The origin of the low steady-state fluorescence quantum yield of some blue-emitting variants of the green fluorescent protein (GFP) is investigated in single-site mutants in which the tyrosine residue at position 66 has been replaced by phenylalanine or by histidine. Time-resolved fluorescence measu