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Photophysical properties of novel fluorescein derivative and its applications for time-resolved fluorescence spectroscopy

✍ Scribed by Mariusz Szabelski; Zygmunt Gryczynski; Ignacy Gryczynski

Book ID: 108109168
Publisher: Elsevier Science
Year: 2010
Tongue: English
Weight: 415 KB
Volume: 493
Category: Article
ISSN: 0009-2614
DOI: 10.1016/j.cplett.2010.05.061

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## Abstract The synthesis of 4(5)‐(bromomethyl)fluorescein, which can be used as its 3′,6′‐dibenzoyl derivative 7 for the labelling of nucleosides is described. Pyrimidine nucleosides such as 3′‐and/or5′‐protected 2′‐deoxyuridine and thymidine are labelled by 7 at N^3^ of the heterocyclic base. The