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Photophysical processes in the NADH—alcohol dehydrogenase complex

✍ Scribed by N.L. Vekshin

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
545 KB
Volume
12
Category
Article
ISSN
1011-1344

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✦ Synopsis

Fluorescent analysis showed the high sensitivity of the NADH-alcohol dehydrogenase complex to UV irradiation. The complex reacts to UV illumination with "photoresponse," a rapid decrease in NADH fluorescence intensity to some stationary level. No such decrease was found in the NADH solution without the protein. The maximum rate of photoresponse was observed at pH 8.0, i.e, the biochemical optimum for the enzyme. It is suggested that the main reason for the photoresponse is desorption of NADH from the protein due to vibrational and conformational relaxation after photoexcitation. The data obtained point to the possibility of controlling "dark" enzymatic processes with light.

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