Photon correlation spectroscopy of hemoglobin: Diffusion of Oxy-HbA and Oxy-HbS
✍ Scribed by C. Ronald Jones; Charles S. Jhonson Jr.; John T. Penniston
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1978
- Tongue
- English
- Weight
- 778 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Translation diffusion coefficients have been measured for oxyhemoglobin A and oxyhemoglobin S over the concentration range 0.1–37 g/dl by means of photon correlation spectroscopy. The solutions were 0.1 M in KCl and in each case the pH was adjusted to the isoelectric point of the hemoglobin species present. No significant differences were found between the HbA and HbS results; and after correction to water at 20°C, the diffusion coefficients could be described by the equation
where D~o~ = (6.93 ± 0.06) × 10^−7^ cm^2^/sec and c ids the concentration in units of g/dl. No evidence was found for the aggregation of oxy‐HbS at high concentrations which was reported by Lindstrom et. al. [(1976) Biophys. J. 16, 679–689].