Photodynamic therapy: in vitro photosensitizing efficacy of bis(tri-n-hexylsiloxy)silicon phthalocyanine against achromic M6 melanocytes. ESR study of active oxygen intermediates
✍ Scribed by Richard Decréau; Angèle Viola; Marie Jeanne Richard; André Jeunet; Michel Julliard
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 186 KB
- Volume
- 02
- Category
- Article
- ISSN
- 1088-4246
No coin nor oath required. For personal study only.
✦ Synopsis
The in vitro photodynamic effect of bis(tri-n-hexylsiloxy)silicon phthalocyanine has been evaluated against the melanotic M6 cell line. The results showed that at 10 À5 M dose, LD 50 is obtained for a 150 J cm À2 light dose and LD 90 for 540 J cm À2 . Electron spin resonance spectroscopy was used with spin traps to study the type I and type II photochemical pathways involved and to detect active oxygen intermediates such as singlet oxygen, oxygen superoxide and hydroxyl radical. The two mechanisms occurred simultaneously and no change was observed when the phthalocyanine was entrapped in liposomes.