Photodynamic effects of methylene blue-loaded polymeric nanoparticles on dental plaque bacteria
✍ Scribed by Vanja Klepac-Ceraj; Niraj Patel; Xiaoqing Song; Colleen Holewa; Chitrang Patel; Ralph Kent; Mansoor M. Amiji; Nikolaos S. Soukos
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 543 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0196-8092
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✦ Synopsis
Background and Objectives: Photodynamic therapy (PDT) is increasingly being explored for treatment of oral infections. Here, we investigate the effect of PDT on human dental plaque bacteria in vitro using methylene blue (MB)-loaded poly(lactic-co-glycolic) (PLGA) nanoparticles with a positive or negative charge and red light at 665 nm. Study Design/Materials and Methods Subgingiva: Dental plaque samples were obtained from 14 patients with chronic periodontitis. Suspensions of plaque microorganisms from seven patients were sensitized with anionic, cationic PLGA nanoparticles (50 mg/ml equivalent to MB) or free MB (50 mg/ml) for 20 min followed by exposure to red light for 5 min with a power density of 100 mW/cm 2 . Polymicrobial oral biofilms, which were developed on blood agar in 96-well plates from dental plaque inocula obtained from seven patients, were also exposed to PDT as above. Following the treatment, survival fractions were calculated by counting the number of colony-forming units.
Results: The cationic MB-loaded nanoparticles exhibited greater bacterial phototoxicity in both planktonic and biofilm phase compared to anionic MB-loaded nanoparticles and free MB, but results were not significantly different (P > 0.05). Conclusion: