Photodynamic Activity of 5,10,15,20-Tetrakis(4-methoxyphenyl)porphyrin on the Hep-2 Human Carcinoma Cell Line: Effect of Light Dose and Wavelength Range
✍ Scribed by Mariángeles La Penna; M.Gabriela Alvarez; E.Ines Yslas; Viviana Rivarola; Edgardo N. Durantini
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 138 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0045-2068
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✦ Synopsis
The photodynamic activity of 5,10,15,20-tetrakis(4-methoxyphenyl)porphyrin (TMP) has been investigated in two systems: reverse micelles of n-heptane/sodium bis(2-ethylhexyl)sulfosuccinate (AOT)/water-bearing photooxidizable substrates and on a Hep-2 human carcinoma cell line. The effect of variation in the light dose and wavelength range (360-800, 455-800, and 590-800 nm) was compared in both media. The aerobic singlet oxygen-mediated photooxidation of L-tryptophan (Trp) was used as a model of biological substrate in a micellar system. A considerable increase of the observed rate constants of Trp (k Trp obs ) was noted, increasing the irradiated area of the TMP spectrum. In vitro, the survival curves of Hep-2 cells, treated with TMP, were markedly dependent on the light wavelength ranges used for irradiation. A linear behavior between k Trp obs and the photoinactivation rate of Hep-2 cells was found, indicating that the singlet oxygen ( 1 O 2 ) is the main species responsible for cell inactivation. These results contributed to an understanding of the photodynamic process yielded by this porphyrin in vitro and the sensitivity of Hep-2 cells to photodamage.