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Photocontrol of affinity chromatography: Purification of asparaginase by photosensitive AHA-gel

✍ Scribed by Isao Karube; Yoshio Ishimori; Shuichi Suzuki; Toshio Sato

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
384 KB
Volume
20
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

An agarose gel modified with N‐(ω‐aminohexytl)‐L‐aspartic acid (AHA) and spiropyran compound (AHA–spiropyran gel) was prepared and the photocontrolled binding and releasing of asparaginase were investigated with the AHA–spiropyran gel. Asparaginase was bound on the AHA–spiropyran gel under visible light and was released in the dark. The optimum conditions for photocontrolled binding and releasing of asparaginase were a 0.05__M__ phosphate buffer concentration and pH 7.0. Seventy‐five percent of the bound asparaginase was released from the AHA–spiropyran gel column in the dark. Ninetyfold purification of asparaginase was performed with the AHA–spiropyran gel Column.

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