Enhancing Fluorescence Brightness: Effec
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Christian Ringemann; Andreas Schönle; Arnold Giske; Claas von Middendorff; Stefa
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Article
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2008
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John Wiley and Sons
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English
⚖ 680 KB
## Abstract Experiments based on fluorescence detection are limited by the population of the fluorescence marker’s long‐lived dark triplet state, leading to pronounced photobleaching reactions and blinking which reduces the average fluorescence signal obtained per time interval. By irradiation with