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Photochemistry of a photosynthetic reaction center immobilized in lipidic cubic phases

✍ Scribed by Alejandro Hochkoeppler; Ehud M. Landau; Giovanni Venturoli; Davide Zannoni; Reiner Feick; Pier Luigi Luisi

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
591 KB
Volume
46
Category
Article
ISSN
0006-3592

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✦ Synopsis

Photosynthetic reaction centers, isolated and purified from the facultative phototrophic bacterium Chloroflexus auranfiacus, were immobilized in optically transparent lipidic cubic phases composed of 42% (w/w) l-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine and 58% (w/w) water. The immobilized photosynthetic protein retains its native properties, a s indicated by visible and circular dichroic spectra. The ground state visible spectrum of the immobilized reaction centers is very similar to the corresponding spectrum in aqueous solution, indicating that the protein pigments are not extracted into the lipidic regions of the cubic phase. The secondary structure of Sons, Inc.

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