Photochemical action spectra of CO-liganded terminal oxidases using a liquid dye laser
✍ Scribed by David Lloyd; Robert I. Scott
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 323 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A method of obtaining photochemical action spectra for the relief of CO inhibition of respiration is described. Continuous readout from a membrane-covered oxygen electrode of dissolved oxygen in a stirred suspension of microorganisms under CO-oxygen gas mixtures in an open reactor enables measurement of increased respiration on illumination. Advantages presented by the use of a liquid dye laser include high intensity of emission and narrow spectral bandwidth; just two dyes (rhodamine 6G and rhodamine 110) are required to match the (Yabsorption maxima of the CO complexes of all known bacterial and mitochondrial terminal oxidases.