Photoautotrophic and photomixotrophic growth of strawberry plantlets in vitro and changes in nutrient composition of the medium

✍ Scribed by T. Kozai; K. Iwabuchi; K. Watanabe; I. Watanabe

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 748 KB
• Volume: 25
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00042181

✦ Synopsis

Explants excised from strawberry (Fragaria x ananassa Duch.) plantlets were cultured in vitro for 21 days on half-strength MS (Murashige & Skoog 1962) basal liquid medium with 20gl 1 sucrose and without sugar in the vessels capped with gas permeable microporous polypropylene film. The experiments were conducted under CO 2 nonenriched (350-450/zmol mol 1 in the culture room) and CO enriched (2,000/xmolmol -I during the photoperiod in the culture room) conditions with a PPF (photosynthetic photon flux) of 200/xmol m-: s -1. The CO 2 concentration in the vessels decreased to approximately 200/xmol mol -~ during the photoperiod on day 21 under CO 2 nonenriched conditions. The fresh and dry weight, net photosynthetic rate (NPR) per plantlet, NPR per g leaf fresh weight, NPR per g leaf dry weight, the number of unfolded leaves, and ion uptake of PO]-, NO3, Ca2+, Mg2+ and K + on day 21 were the greatest under photoautotrophic (no sugar in the medium) and CO enriched conditions. The residual percent of PO]-was 3% on day 21 under photoautotrophic and CO enriched conditions.