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Photoaffinity labelling of the nucleoside transporter of cultured mouse lymphoma cells

โœ Scribed by Anthony F. Almeida; Simon M. Jarvis; James D. Young; Alan R.P. Paterson


Book ID
115916207
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
453 KB
Volume
176
Category
Article
ISSN
0014-5793

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A mutant clone (NT-1) of a T-cell lymphoma was selected for its ability to grow in HAT medium (hypoxanthine, aminopterin and thymidine) in the presence of the nucleoside transport inhibitor P-nitrobenzyl-6-mercaptoinosine (NBMI). NT-1 cells contain half the number of NBMI binding sites present on th