𝔖 Bobbio Scriptorium
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Photoaffinity labelling of cardiac membrane GTP-binding proteins in response to insulin

✍ Scribed by Martina RUSS; Antje WICHELHAUS; Ingo UPHUES; Thomas KOLTER; Jürgen ECKEL

Book ID
115130996
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
863 KB
Volume
219
Category
Article
ISSN
1432-1327

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The mechanism of steroid uptake by the cell remains controversial. [3H]R5020 was utilized to characterize by photoaffinity labeling the steroid binding site in plasma membrane. This binding was saturable, reversible and had one type of binding site (Kd = 33 +/- 4 nM, Bmax = 32 +/- 2 pmol/mg). [3H]R5