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Photoaffinity labeling of the adenosine cyclic 3',5'-monophosphate receptor protein of Escherichia coli with 8-azidoadenosine 3',5'-monophosphate

✍ Scribed by Aiba, Hiroji; Krakow, Joseph S.


Book ID
126860273
Publisher
American Chemical Society
Year
1980
Tongue
English
Weight
617 KB
Volume
19
Category
Article
ISSN
0006-2960

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An earlier report (la) has shown the utility of 8-N3 cAMP (8-azidoadenosine-3', 5'-cyclic monophosphate) as a photoaffinity probe for cAMP binding sites in human erythrocyte membranes. The increased resolution obtained using a linear-gradient SDS polyacrylamide gel system now shows that: 1) both cAM