Photoaffinity labeling of the adenosine cyclic 3',5'-monophosphate receptor protein of Escherichia coli with 8-azidoadenosine 3',5'-monophosphate
β Scribed by Aiba, Hiroji; Krakow, Joseph S.
- Book ID
- 126860273
- Publisher
- American Chemical Society
- Year
- 1980
- Tongue
- English
- Weight
- 617 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0006-2960
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Rates of synthesis of cyclic 3',5'-adenosine monophosphate (cAMP) were measured in cultures of Escherichia coli aerating without a carbon source. This technique provides a representative measure of adenylate cyclase activity in the absence of inhibition caused by transport of the carbon source. Aden
An earlier report (la) has shown the utility of 8-N3 cAMP (8-azidoadenosine-3', 5'-cyclic monophosphate) as a photoaffinity probe for cAMP binding sites in human erythrocyte membranes. The increased resolution obtained using a linear-gradient SDS polyacrylamide gel system now shows that: 1) both cAM