𝔖 Bobbio Scriptorium
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Photoaffinity Labeling of Steroid Binding Proteins with Unmodified Ligands

✍ Scribed by Hannes M. WESTPHAL; Gerhard FLEISCHMANN; Miguel BEATO

Book ID
115120197
Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
566 KB
Volume
119
Category
Article
ISSN
1432-1327

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The mechanism of steroid uptake by the cell remains controversial. [3H]R5020 was utilized to characterize by photoaffinity labeling the steroid binding site in plasma membrane. This binding was saturable, reversible and had one type of binding site (Kd = 33 +/- 4 nM, Bmax = 32 +/- 2 pmol/mg). [3H]R5