Photoaffinity labeling of bovine rhodopsin
โ Scribed by Ranjan Sen; Anil K. Singh; Valeria Balogh-Nair; Koji Nakanishi
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- French
- Weight
- 834 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0040-4020
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
We report a rapid and high-yield purification method of bovine retinal rhodopsin kinase. According to our method, 500 micrograms of rhodopsin kinase was purified from 100 bovine retinae within 12 h. Rhodopsin kinase bound to bleached rhodopsin was extracted effectively from rod outer segment membran
## Dedicated to Professor Albert Eschenmoser on the occasion of his 75th birthday The retinal chromophore (11Z)-3-diazo-4-oxoretinal (1) with two photo-labile moieties has been synthesized by semi-hydrogenation of an 11-yne precursor with activated Zn in aqueous media. Incorporation of 1 into opsi
## Abstract The amino terminus of bovine rhodopsin is blocked and has the sequence xโMetโAsn(CHO)โGlyโThrโGluโGlyโProโAsnโPheโTyrโValโProโPheโSerโAsn(CHO)โLysโThrโGlyโValโValโArg, where CHO represents sites of carbohydrate attachment. The carboxylโterminal sequence of rhodopsin is ValโSerโLysโThrโG