𝔖 Bobbio Scriptorium
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Photoaffinity Labeling of Anion Transport Components in Hepatocyte Plasma Membranes

✍ Scribed by Daniel Levy; Shirley Cheng


Book ID
118718393
Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
531 KB
Volume
346
Category
Article
ISSN
0890-6564

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The mechanism of steroid uptake by the cell remains controversial. [3H]R5020 was utilized to characterize by photoaffinity labeling the steroid binding site in plasma membrane. This binding was saturable, reversible and had one type of binding site (Kd = 33 +/- 4 nM, Bmax = 32 +/- 2 pmol/mg). [3H]R5