𝔖 Bobbio Scriptorium
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Photoaffinity labeling of a novel benzodiazepine binding protein in rat brain

✍ Scribed by Allen C. Bowling; Robert J. DeLorenzo


Book ID
115881160
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
307 KB
Volume
135
Category
Article
ISSN
0014-2999

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The mechanism of steroid uptake by the cell remains controversial. [3H]R5020 was utilized to characterize by photoaffinity labeling the steroid binding site in plasma membrane. This binding was saturable, reversible and had one type of binding site (Kd = 33 +/- 4 nM, Bmax = 32 +/- 2 pmol/mg). [3H]R5