Photoaffinity labeling and characterization of a juvenile hormone binding protein in the membranes of follicle cells of Locusta migratoria
β Scribed by Veeresh L. Sevala; K.G. Davey; Glenn D. Prestwich
- Book ID
- 116169376
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 884 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0965-1748
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The mechanism of steroid uptake by the cell remains controversial. [3H]R5020 was utilized to characterize by photoaffinity labeling the steroid binding site in plasma membrane. This binding was saturable, reversible and had one type of binding site (Kd = 33 +/- 4 nM, Bmax = 32 +/- 2 pmol/mg). [3H]R5
Earlier work had shown that JH acts on the membrane of the follicle cell of Locusta migratoria, bringing about a rapid reduction in volume which can be detected in vitro by measuring the increase in optical path difference using quantitative interference microscopy. The juvenoid fenoxycarb, a phenox